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Image Search Results
Journal: Oncotarget
Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells
doi: 10.18632/oncotarget.12720
Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Article Snippet: GDNF levels were determined by using a Human glial cell line-derived
Techniques: Cell Culture, Quantitative RT-PCR, Western Blot
Journal: Oncotarget
Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells
doi: 10.18632/oncotarget.12720
Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.
Article Snippet: GDNF levels were determined by using a Human glial cell line-derived
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics
Journal: Journal of Neural Engineering
Article Title: Enhancing facial nerve regeneration with scaffold-free conduits engineered using dental pulp stem cells and their endogenous, aligned extracellular matrix
doi: 10.1088/1741-2552/ad749d
Figure Lengend Snippet: Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using ELISA, indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor (GDNF) in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Article Snippet: Then human BDNF (PicoKine ELISA Kit, Boster Biological Technology) and
Techniques: Staining, Light Microscopy, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Immunofluorescence, Produced